Globally, anthropogenic activities such as targeted fishing and habitat modification have posed great threat to elasmobranchs. As species’ resilience to these pressures is largely determined by key life-history parameters, and elasmobranchs tend to exhibit low fecundity, late maturation and slow growth, it has become apparent that knowledge of reproductive characteristics and mating strategies is important for accurately monitoring populations. Whilst the anatomy and embryonic development modes of elasmobranchs are relatively well characterised, little is known of the link between reproductive mode and reproductive strategy (e.g., multiple paternity), or how this may be influenced by a species’ ecology. To date, multiple paternity has been identified in seven elasmobranch orders. Of this, the carcharhinids (Carcharhinidae) are the most represented family, with nine out of 11 assessed species confirmed to exhibit some degree of multiple paternity. The spinner shark (Carcharhinus brevipinna) is a wide-ranging species of carcharhinid, and is an important component of commercial fisheries worldwide. In this study, we provide the first assessment of multiple paternity in this species, through an analysis of single nucleotide polymorphisms (SNPs) drawn from two mother-litter groups from the coast of southeast Queensland, Australia. Given the paucity of reproductive information on this species, this research advances our knowledge of this species and may aid to advance effective conservation and management of this commercially important species.